I hypothesize that increasing concentrations of magnesium will positively affect the growth of V. Radiata. The greater the concentration of the magnesium solution, the greater the growth of the plants. The fertilized plants will have greater growth than the non-fertilized because the combination of fertilizer and Epsom salts will negate more of the impact that the initial lack of magnesium in the topsoil will cause.
In this investigation, the independent variable is the magnesium concentration. Different concentrations of magnesium will be tested to see how they affect the length and weight of the plant (dependent variable). The 6 different concentrations that will be used are 0%, 1.27%, 2.53% 5.05%, 10.10% 20.19%.
In this investigation, the dependent variable is the growth rate of the Vigna radiata seeds as they are reliant on the magnesium concentration (independent variable). Magnesium deficiencies reduce plant growth and the length and weight will be quantified.
A control variable in a scientific investigation is a variable that is kept constant throughout the experimentation period. It is held constant in order to test the relationship between the independent and dependent variables. If failed to be held constant, the controlled variables would invalidate the correlation between the independent and dependent variables. The control variables in this experiment are amount of light, temperature, water, seeds, Epsom salts, soil and fertilizer.
Light – The twelve pots will be kept close together, surrounded by twelve lamp lights, to ensure that the seeds receive the same amount of light. Although it cannot be calculated, it will remain constant because of the strategic arrangement of pots and lamp lights. This will ensure that the correlation between magnesium concentrations and plant growth.
Temperature – The twelve pots will be kept close in order to assure that the temperature in the investigation area remains the same. The temperature will be 23OC ± 0.5. Thus, the variation of a pot remains in conjunction with the temperature of the other pots. Therefore, temperature will not affect the relationship between magnesium concentrations and plant growth.
Water – Initially, two L of water will be given to the soil in the pots in order to simulate a flooding. The water used is distilled and bought from the same vendor to guarantee that homogenous characteristics are present and no impurities fluctuate.
Seeds – The mung bean seeds were bought from the same vendor because different vendors may genetically modify seeds to ensure a higher yield.
Epsom salts & Soil and Fertilizer – The Epsom salts, soil and fertilizer are all bought from the same vendor. Buying materials from different vendors can result in varying physical properties which would reduce the validity between the independent and dependent variables.
CHAPTER II: PROCEDURE
In this investigation, the pots will be put into two groups, Group A will not be provided fertilizer, group B will be. A magnesium deficiency will be caused in the pot soil and fertilizer by providing lots of water, this will result in magnesium, which is water-friendly, to go to the lower levels of the soil. During the investigation phase, the plants of each group will be given different concentrations of Magnesium (Mg2+), the varying concentrations will be injected using aqueous Epsom salts. Aqueous Epsom salts are magnesium sulfate (MgSO4), they have a molar mass of 120.366 g/mol. The element magnesium has a molar mass of 24.305. Thus, the magnesium concentration in the stock solution is 20.19%. The stock solution will be diluted to the different concentrations, using serial dilutions. The magnesium concentrations for the Epsom salts that will be used are 0%, 1.27%, 2.53% 5.05%, 10.10% 20.19%.
To thoroughly investigate how a magnesium deficiency affects plant growth, and whether fertilizer can negate some of the effects, this investigation will be split into two parts. Part one is a two-week long period, after the two weeks, the plants will be physically removed from the soil and qualitative observations will be taken on root conditions. In part two, the entire procedure will be repeated and the investigation portion will carry on for four weeks. The purpose of splitting this investigation into two parts is to clearly see the correlation between magnesium concentrations and plant growth. In addition, examining root conditions and plant length and weight in the early stage as compared to plant length and weight in the later stage, will highlight whether a lack of magnesium has a greater effect during the early or later stage of growth.